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Integrating Spheres and Fluorescence Quantum Yield

Fluorescence Quantum Yield measurements provide information about the efficiency of a molecule’s fluorescence and is defined as the ratio of the number of photons absorbed to the number of photons emitted by a sample. Different molecular and environmental conditions not only effect whether a molecule will fluoresce or not, but can also determine the intensity of the emitted fluorescence radiation. Fluorescence quantum yield is measured using an integrating sphere to ensure that all sample fluorescence is collected

Refelctance
Integrating Spheres
ISF-834 60 mm Integrating Sphere
Integrating Spheres

For quantum efficiency and color evaluation measurements of opaque solid or powder samples.

ILF-835 100 mm Integrating Sphere
Integrating Spheres

For quantum efficiency measurements of liquids or thin membrane samples on a transparent substrate as well as opaque solid or powder samples.

ILFC-847 Liquid N2 Cooled 100 mm Integrating Sphere
Integrating Spheres

Cools samples with liquid nitrogen for fluorescence quantum yield and phosphorescence lifetime measurements. It can also be used at ambient temperatures without liquid nitrogen.

Cells and Cell Holders for Integrating Spheres
Cells and Cell Holders
Cell Holders Cell Holders Cell Holders

1 mm Liquid Cell

1 x 10 mm path length, 2 mL sample volume

2 mm Liquid Cell

2 x 10 mm path length, 400 mL sample volume

3 mm Powder Cell

19 (H) x 10 (W) x 3 (T) mm

10 mm Rectangular Cell Holder

Used to mount a 10 x 10 mm rectangular cell inside the ILF-835/ILFC-847 integrating spheres.

KBr Plate Sample Holder

Used to sandwich a powder sample between two KBr plates (5 × 5 × 1 mm). The KBr sample holder can also be used for micro FTIR measurements.

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