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HPLC and UHPLC Systems

LC-4000 Series Integrated HPLC and UHPLC Systems

The LC-4000 Series is the latest in a long history of innovative HPLC systems developed by JASCO, reaching all the way back to the start of commercial HPLC in the early 1970s. The concept of the integrated LC-4000 series HPLC provides key separation platforms at 50MPa, 70MPa, and 130MPa which correspond to conventional HPLC, the increasingly popular Rapid Analysis (RHPLC), and sub 2um UHPLC respectively.

Advances in Solvent Delivery

For more nearly three decades JASCO analytical HPLC pumps have employed an asymmetric twin-piston solvent delivery system SSQD (Slow Suction, Quick Delivery) which provides significantly better flow and pressure profiles than other conventional twin-piston reciprocating design. In the LC-4000 Series, SSQD has been re-developed, with a completely new solvent delivery mechanism ExReFT (Extremely Reliable Flow Technology) offering the highest stability in solvent delivery across the entire analytical flow rate range used in the PU-4180/85 RHPLC and PU-4280/85 UHPLC pump models.

Pioneering Optical Design

As a pioneer in optical spectroscopy, JASCO has access to optical designs that few can match. Taking advantage of our more powerful spectrometers, we have developed a range of HPLC detectors with unrivaled performance, like the new class leading FP-4020 fluorescence detector ( water Raman S/N > 2300:1, the latest high-speed refractive index detector with micro cell optimized for UHPLC and the unique circular dichroism detector for chiral chromatography.

New Technologies for Method Development

With the range of column technologies becoming increasingly diverse – the traditional 5μm fully porous particle is finally losing ground to some of the newer technologies such as the new superficially porous solid core (Coreshell) and the wide variety of sub-2μm particles – the requirements for method development are becoming more demanding. Designed to accommodate analytical, preparative and multiple columns at every scale with options for integrated 6 or 10 solvent and column selection valves. The LC-4000 Series has a number of newly developed accessories to make better separations faster.

Compact and Easy to Use

Despite the extra power delivered by the LC-4000 Series HPLC, the standard footprint is only 300 mm wide – a standard system requires only a very small amount of bench space. For easy maintenance, all LC-4000 instruments feature front-panel access for replacing consumables such as pump components and light sources. The autosampler consumables such as the syringe parts are easy to access. For users requiring front-panel control, the LC-4000 Series retains a keypad and backlit LED display. Keypad control can still be configured for convenient override when under PC control.


Front-panel access to light source and consumables

Each LC-4000 module is designed for easy maintenance. The LC-4000 Series pump module requires smaller bench space than ordinary HPLC pumps. An integrated solvent delivery system can be incorporated into a single pump module.

Common Features of the LC-4000 Analytical and Preparative Systems
  • 300 mm wide footprint
  • Each system component is completely stackable into a modest height
  • Fully integrated with complete system monitoring
  • All user replaceable components are accessible through the front panel
  • Vertical leak/drain system to channel away solvent
  • Integrated earthquake securing option
  • Multiple pump control for up to 4 gradient pumps in a single system
  • Up to 5 detectors and 3 columns ovens
  • 6 or 10 column and solvent selection for method development
  • All detectors include 100 Hz acquisition with interchangeable flow cells
HPLC Systems
Conventional HPLC and RHPLC Analytical Systems up to 70MPa

The LC-4000 Series 70 MPa system has been designed to future-proof your HPLC requirements. The LC-4000 70 MPa system can be used with conventional 3 and 5 μm particle size analytical columns at typical lower pressures around 10 to 20 MPa (1500 to 3000 psi) and can also be used with smaller particles such as shorter length UHPLC columns and superficially porous (SPP) or Coreshell that require slightly higher pressure solvent delivery to provide optimum linear velocity through the column.

UHPLC Analytical Systems at 130MPa

Ten years after the new segment of the HPLC market, UHPLC has found a niche for those that need to run a large number of samplers or get results quickly. Pioneering column technology is pushing the particle size even smaller. The near 20,000 psi operation of the LC-4000 130 MPa is designed to take advantage of the separation efficiency of the very small particles couple with longer and narrower columns. The LC-4000 130 MPa system incorporates many of the same features as the 70 MPa system, but with materials designed to withstand higher pressures.

Preparative HPLC Systems

The LC-4000 preparative HPLC systems includes three platform options:

  • Up to 20 mL/min for columns up to 21.2 mm ID
  • Up to 50 mL/min for columns up to 3 cm ID
  • Up to 120 mL/min for columns up to 5 cm ID

Large column oven for multiple preparative columns and optional automatic column switching
Open-bed fraction collector with sample trays for tubes, plates or large containers
ChromNAV-FC for fraction control using time, threshold or slope from up to 4 different signals

HPLC/UHPLC application notes

Click on the applications below for more information

  1. Preparative HPLC and Fraction Collection
  2. Anion Analysis using Ion Chromatography with Chemical Suppression
  3. Analysis of Nylon 6 by Semi-micro Scale GPC
  4. Analysis of Sugar Alcohols by Semi-micro HPLC
  5. Analysis of Polystyrene Oligomers by Semi-micro GPC
  6. Measurement of warfarin using circular dichroism coupled with HPLC
  7. Chiral Analysis of Flurbiprofen using a Circular Dichroism Detector
  8. Chiral analysis of Ibuprofen using a Circular Dichroism Detector
  9. Analysis of Fatty Acids using the LC-4000 Series Rapid Separation HPLC
  10. Detection of Amino Acids Using HPLC with CD Detection
  11. Analysis of Aldehydes using Post-Column Derivatization by High Performance Liquid Chromatography
  12. Water Analysis for Aldehydes using Post-column Derivatization by HPLC
  13. High Speed Analysis of Melamine in Dairy Products by HPLC
  14. Analysis of Sugars by HPLC-ELSD
  15. Analysis of Sugar Alcohols by HPLC-ELSD
  16. Analysis of p-Hydroxybenzoate Esters by HPLC-ELSD
  17. Analysis of Nonionic Surfactants by HPLC-ELSD
  18. Analysis of Formaldehyde in Air using Post-column Derivatization by HPLC
  19. Analysis of Fatty Acids in Rice Bran Oil, Coconut Oil and Margarine by HPLC-ELSD
  20. Analysis of Dabsyl Amino Acids using HPLC
  21. Analysis of Cyclodextrines in Green Tea using HPLC-ELSD
  22. Analysis of Anti-flatulent Compounds using HPLC-ELSD
  23. Analysis of Protein Hydrolysate Amino Acids using OPA Post-column Derivatization by Quaternary Low Pressure Gradient System
  24. Analysis of Triglycerides by High Performance Liquid Chromatography with Evaporative Light Scattering Detection
  25. Analysis of Benzoic Acid, Sorbic Acid and Dehydroacetic Acid
  26. Analysis of Amino Acids by Automated Pre-column Derivatization with OPA
  27. Analysis of Allantoin
  28. Analysis of Total Aflatoxins in Food by HPLC and UHPLC
  29. Semi-preparative Separation of Glycyrrhizic Acid in Glycyrrhiza
  30. Semi-preparative Separation of Ginsenoside in Ginseng
  31. Semi-preparative Separation of Berberine in Coptis Japonica
  32. Analysis of Capsaicin and Dihydrocapsaicin in Capsicum
  33. Recycling Preparative HPLC of Polystyrene Oligomer
  34. An evaluation of physico-chemical properties of the mobile phase in Supercritical Fluid Chromatography when using sub-2µm particle columns
  35. Sugar Analysis in Tea using Optical Rotation and Refractive Index detection
  36. Analysis of Chondroitin Sulfate by Size Exclusion Chromatography
  37. Analysis of p-Hydroxybenzoate Ester
  38. Analysis of Saccharin Sodium
  39. Non-ionic Surfactant Analysis by Solid-phase Extraction
  40. Simultaneous Determination of Bile Acids Utilizing an Immobilized Enzyme Column
  41. Detection of Nitroarenes in Diesel Exhaust Using HPLC with UV and Fluorescence Detection
  42. An Evaluation of Four Commercial HPLC Chiral Detectors: A Comparison of Three Polarimeters and a Circular Dichroism Detector
  43. Ion Chromatography of Chlorate Ions in Tap Water
  44. Detection of Chiral Drugs Using HPLC with CD Detection
  45. HPLC of Fatty Acid Methyl Ester (FAME) and Triglyceride in Biodiesel and Diesel
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