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V-750 UV-Visible Spectrophotometer

The V-750 is a high resolution UV-visible double-beam spectrophotometer with single monochromator, variable spectral bandwidth and a photomultiplier tube (PMT) detector.

The optical design uses precision double-beam optics with variable spectral bandwidth. A high sensitivity PMT provides accurate and reproducible measurements for low to high concentration samples. By controlling the high voltage applied to the PMT, the dynode feedback offers a wider dynamic range. For high resolution measurements, such as gas and vapor phase spectroscopy the spectral bandwidth can be set as narrow as 0.1 nm. Special low stray light slit settings which optimize the light image on the monochromator provide increased linearity to up to 5 absorbance units.
With a comprehensive range of sampling accessories, the V-750 is well suited to measurement of solid and liquid samples in the UV and visible regions, with temperature control for biological samples and a number of transmittance and reflectance accessories for materials analysis.

UV Spectrophotometers
Operating Systems

Two user interfaces are available. Spectra Manager™ Suite the innovative cross-platform spectroscopy software, compatible with Windows 7 Pro (32- and 64-bit) and Windows 8.1 operating systems.
For simple operation, the intelligent remote module (iRM) has a new look and feel, with a color LCD touch screen. Data can be downloaded for portability or further PC data processing using Spectra Analysis.
The V-700 Series of UV-visible spectrophotometers has a growing list of software applications for both Spectra Manager™ and for the iRM. If you require an application which you don’t see listed, please let us know as we may already have it or we can prepare an application designed specifically for your requirements.
Both interfaces allow full system control and advanced data processing. Spectra Manager™ CFR is the 21 CFR part 11 compliant version of software and is available as an option.

IQ Accessory and IQ Start

IQ Accessory provides automated accessory recognition with automatic loading of a user defined method. Spectral data files include the instrument and accessory information while designated instrument parameters can be configured and loaded for optimum sample measurements. IQ Start together with the Start Button is used to start a sample method for immediate access when making routine measurements.

Optical Performance

The most recent advances in optical technology have been used to provide excellent instrument reliability and assure highly accurate results. High throughput optics combined with the latest in electronic design provide excellent sensitivity and stability for all sample measurements.

Sampling Accessories

An extensive range of accessories is available for the V-700 Series of UV-Visible/NIR spectrophotometers for virtually any biological, material science or routine QA/QC measurement. Some accessories are common across the V-700 Series and others are instrument specific. Refer to the instrument brochure for accessories specific to this instrument.
Options include a wide variety of liquid cell holders, micro and ultra-micro cell holders, flow cell holders and accessories for solid samples. Advanced accessories, including automated cell changers, sippers, integrating spheres, automated and manual absolute reflectance accessories and programmable temperature control systems are available for any of the V-700 instrument systems.

Instrument Validation and Regulatory Compliance

The V-750 includes user-selectable validation to USP, EP and JP (or manually editable). With optional IQ/OQ.

V-750 UV-Vis Spectrophotometer Specifications

Optical System Czerny-Turner mount
Single monochomator
Fully symmetrical double beam type
Light source Halogen lamp, Deuterium lamp
Wavelength range 190 to 900 nm
Wavelength accuracy +/-0.2 nm (at 656.1 nm)
Wavelength repeatability +/-0.05 nm
Spectral bandwidth (SBW) 0.1, 0.2, 0.5, 1, 2, 5, 10 nm
L2, L5, L10 nm (low stray light mode)
M1, M2 nm (micro cell mode)
Stray light 1 % (198 nm KCL 12 g/L aqueous solution)
0.005 % (220 nm NaI 10 g/L aqueous solution)
0.005 % (340 nm NaNO2 50 g/L aqueous solution)
0.005 % (370 nm NaNO2 50 g/L aqueous solution)
SBW: L2 nm
Photometric range -4~4 Abs
Photometric accuracy +/-0.0015 Abs (0 to 0.5 Abs)
+/-0.0025 Abs (0.5 to 1 Abs)
+/-0.3 %T
Tested with NIST SRM 930D
Photometric repeatability +/-0.0005 Abs (0 to 0.5 Abs)
+/-0.0005 Abs (0.5 to 1 Abs)
Tested with NIST SRM 930D
Scanning speed 10~4000 nm/min (8000 nm/min in preview mode)
Slew speed 12,000 nm/min
RMS noise 0.00003 Abs
(0 Abs, wavelength: 500 nm, measurement time: 60 sec, SBW: 2 nm)
Baseline stability 0.0003 Abs/hour
(Wavelength: 250 nm, response: slow and SBW: 2 nm)
Baseline flatness +/-0.0002 Abs (200 - 850 nm)
Detector Photomultiplier tube
Standard functions IQ accessories, Start button, Analog output
Standard programs Abs/%T meter, Quantitative analysis, Spectrum measurement, Time course measurement, Fixed wavelength measurement, Validation, Daily check, Two wavelength time course measurement
Dimensions and weight 460(W) x 602(D) x 268(H) mm, 27 kg
Power requirements 150 VA
Installation requirements Room temperature: 15-30 Celsius, humidity: below 85%
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