One Drop - Low Volume

Precise analysis of micro-volume samples for biological samples

The SAH-769 One Drop is a dedicated accessory for the V-600 and V-700 Series UV-visible spectrophotometers for the measurement of micro-volume samples of protein and nucleic acid. A precise optical pathlength is maintained, assuring excellent measurement reproducibility. The sampling procedure is very quick (around 20 seconds per sample) and easy, by simply placing a droplet of a sample on the disk cell window. The minimum sample volume is 5 µl for the standard 1 mm pathlength disk cell, and 0.6 µL for the optional 0.2 mm pathlength cell. The shorter optical pathlength allows measurements of high concentration samples without dilution.

Measurement

Rapid, but highly accurate measurement using a flexible V-700 spectrophotometer. Only 20 seconds per sample.

Quantitative analysis of a 0.6 µl sample of calf thymus DNA using a 0.2 mm pathlength disk cell

Spectra of calf thymus DNA solutions

Calibration curve of calf thymus DNA. The figure illustrates excellent linearity up to approx. 3000 ng/µL. This is equivalent to a linearity of up to 35 Abs for a 10 mm pathlength cell.

EMC-709 Micro Cell Holder

The EMC-709 is a micro cell holder for micro volume cells (approx. 50 µL or smaller). For use with rectangular micro cells with a path length up to 10 mm and a width of 2 mm, minimum.


Compatible Cell	Light path length Light path width Bottom thickness	10 mm 2 mm 2.5 mm or less
Recommended Cell (Optionally available)	1103-0114A 1103-0194A 1103-0201A 6708-J046A	50 µL Micro cell 5 µL Micro cell (Normal cell) 5 µL Micro cell (Single sided cell) Spacer for 5 µL Micro cell

50 µL Micro cell

Light path length: 10 mm
Light path width: 2 mm
Sample Volume: 50 µL
P/N: 1103 – 0114A
(Two sides are black quartz)

5 µL Micro cell (Normal type)

Light path length: 1 mm
Light path width: 2 mm
Sample Volume: 5 µL
P/N: 1103 – 0194A

5 µL Micro cell (Single sided type)

Light path length: 1 mm
Light path width: 2 mm
Sample Volume: 5 µL
P/N: 1103 – 0201A

Spacer for 5 µL Micro cell

After inserting a 5 µL Micro cell into the spacer, it is placed into the sample holder.
P/N 6708 - J046A

EMC-759 5 µL Ultra-micro cell holder

The EMC-759 micro cell holder for micro volume cells (approx. 5 µL) can be used for minimal volume samples. Rectangular micro cells with a path length of 1 mm and a width of 2 mm are specified for use with this cell holder, using a lens to focus the light beam into the cell for precise low-volume measurements.


Compatible Cell	Light path length Light path width Bottom thickness	1 mm 2 mm 3.5 mm or less
Recommended Cell (Optionally available)	1103-0194A 1103-0201A	5 µL Micro cell (Normal cell) 5 µL Micro cell (Single sided cell)

Due to the optical path the EMC-759 is designed only for use with the V-630 and V-730 UV-Vis instruments (incl. Bio models) For other V-series instruments, please select the EMC-709 cell holder.

5 µL micro cell (stepped type)

This micro-volume cell (5 µL volume) is designed so that a pipette tip can reach the cell bottom and sample recovery can be easily performed. The dedicated cell holder, model EMC-759, is required to use this cell and the light beam is condensed using a lens integrated into the cell holder. Optimum measurements are obtained by reducing the source beam energy losses due to diffraction.

TCH-703 8-position micro turret cell holder. Minimum sample volume: 4 µL

The TCH-703 turret cell holder is designed for use only with the V-630/V-730 (incl. Bio models). The turret cell holder contains a ‘turret’ with 8 micro cells of 1 mm pathlength with a minimum sample volume of 4 µL each.
The cell position is manually selected with a guaranteed path length for reproducible results.
An optimized optical system reduces source beam energy losses due to diffraction of the incident beam. This optical system provides reproducible measurement of dilute and micro samples.
The turret can be removed for easy cleaning and loading of samples.