MD-4010 PDA

The LC-4000 Series includes three PDA detectors. The MD-4017 is a useful replacement for a single wavelength detector, the MD-4015 is the workhorse for UV- visible detection in the range 200 to 600nm and the MD-4010 is designed for high sensitivity and high spectral and time resolution.

The MD-4010 PDA detector offers the highest detection sensitivity, equivalent to a single wavelength detector. Making use of a 1024 element photometric diode array, the measurement wavelength range is from 190nm to 900nm with a MD-4010 PDA resolution of 1nm and fast spectrum acquisition rate of up to 100 spectra/sec. When used with the new temperature controlled SP type semi-micro flow cell the MD-4010 is well suited to the narrower peaks typically found with UHPLC or RHPLC separations. The MD-4010 is fitted with an internal mercury lamp for automatic wavelength calibration.

The MD-4015 PDA detector is the routine detector for conventional HPLC, RHPLC and UHPLC. The sensitivity is almost equivalent to that of the MD-4010 but only covers the wavelength range from 200nm to 600nm using a 512 element PDA for 1nm resolution and with high speed 100Hz spectrum acquisition.

The MD-4017 detector has a price-point comparable to a typical single wavelength detector and has been designed for the user only interested in the UV region from 200-400nm, the resolution is excellent at 1nm and the maximum spectrum acquisition is 20 spectra per second (20Hz), useful for peak widths as small as 1 to 2 seconds.
When used with ChromNAV 2.0 CDS the PDA detector offers multi-functional analysis with a variety of functions such as spectral analysis, library search for peak identification, peak purity and multi-wavelength quantitation.

UV-4070 UV-Visible Detectors

Using the advanced Czerny- Turnermonochromator design with optimization of the optical system, baseline change due to refractive index is minimized. Baseline fluctuation from changes in environmental temperature is reduced with a temperature - controlled cell, baseline drift caused by temperature fluctuation in the light source is also reduced with a temperature controlled light source. The result is excellent stability with UV-4070 UV Visible UV spectra auto-scan Automatic spectral scannning can be controlled using ChromNAV, when a peak exceeds a set threshold a scan is executed automatically. 0.24 220000 200000 the noise level +/- 0.2x10 -5 AU and drift 1x10 -4 AU/h.

Both detectors offer simultaneous dual-wavelength data acquisition and spectral scanning. User maintenance can be done without removing the module from the system. Front-panel access is used for light source replacement and automatic wavelength calibration can be carried out using a mercury lamp. Both detectors include high speed data acquisition at up to 100Hz and can be used for conventional HPLC with the standard flow cell and for UHPLC/RHPLC when used with the SP type semi-micro cell.

FP-4020 Fluorescence

The FP-4020 fluorescence detector has been designed for the pursuit of extreme sensitivity. Re-designing the optics and electronics from the ground up, as well as removing the effects of ambient temperature with a new temperature- regulated cell body has led to the highest sensitivity with stable detection. The new axially irradiated cell design enables the very highest sensitivity detection. These changes have resulted in a giant leap in sensitivity for the FP-4020 to achieve a signal noise ratio of 2300:1 or higher (based on the water Raman peak). The FP-4025 is an excellent routine fluorescence detector that has a slightly lower specification with a water Raman S/N of 1400:1.

Both detectors can be used for simultaneous detection at two wavelength pairs; excitation and emission wavelengths can be changed during the acquisition using a time program and automatic spectra scanning can be triggered manually, by time or automatically by threshold. A built-in mercury lamp enables automatic wavelength correction and the front-access design allows easy replacement of the flow cell assembly and light source.

High sensitivity measurement of Anthracene

Dual wavelength simultaneous measurement

RI-4030 Refractive Index

The Refractive Index detectors include the optical system mounted in a precisely temperature-regulated housing to eliminate the influence of changes in ambient temperature. The RI-4030 and 4035 detectors are fully stable just one hour after powering on. The RI-4030 can be used for both analytical and preparative scales. In preparative mode the maximum flow rate is 120 ml/min. The RI-4035 is a dedicated semi-micro RI detector with a micro-volume flow cell designed for use with semi-micro GPC analysis using GPC columns with a fine pore size.

The 100Hz data acquisition rate makes the RI-4035 suitable for RHPLC and UHPLC separations. The LED light source in both detectors has a long life time (over 10,000 hours) for low maintenance operation. Both the RI-4030 and RI-4035 detectors can be fitted with an optional pressure release safety-valve to prevent damage to the flow cell in the event blockage.

Circular Dichroism detector CD-4095

The CD-4095 is a unique HPLC detector for measuring circular dichroism developed from the market leading J-Series circular dichroism spectropolarimeter. Optically active compounds with a chromophore close to a chiral center may absorb circularly polarized light, which can be detected with excellent sensitivity and selectivity. When chiral compounds are measured using a UV-Visible detector, the d- and l- enantiomers cannot be distinguished even when separated by a chiral column.

The CD-4095 circular dichroism detector makes it possible to positively distinguish between the d- and l- enantiomers by measuring the positive and negative peaks. It is also possible to quantify the d- and l-forms even when they are not separated, a calibration curve based on the g-factor can be used to determine the enantiomeric ratio. The ChromNAV CDS can be used to simultaneously measure the CD, g-factor data and UV-visible signal. The CD spectra of eluted compounds can be measured by triggering scanning manually, by timed program or automatically by threshold.