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Fluorescence Stopped Flow and Automated Titration

Fluorescence Stopped Flow/Rapid Kinetics
Stopped Flow

The Fluorescence Stopped Flow accessory is used with either the FP-8300 or FP-8500 for measurement of rapid kinetic events by mixing two or more solutions to initiate a fast chemical reaction with very small delay time. The reaction kinetics are then followed by monitoring the change in the fluorescence intensity.

Model SFS-852 SFS-853 SFS-854 SFS-852T SFS-853T SFS-854T
Number of Syringes 2 3 4 2 3 4
Syringe Volume Options 10 mL (standard), 1.0, 2.5, 5.0 mL
Mixing Ratio 1:1 to 1:20
Dead Time 2.9 msec.
Flow Rate 5 mL/sec. (10 mL syringe)
Temperature Control System - - - Heating/Cooling system utilizing Peltier effect
Peltier Heat Radiation - - - Water-Cooled
Temperature Setting Range - - - Cell: 10 to 80°C; Syringe: 10 to 60°C
Temperature Accuracy - - - ±0.5°C (cel)
Optional Accessory 50 μL, 100 μL, 500 μL delay line
Automated Titration
Autosampler

The dual syringe autotitrator is used to monitor changes in the fluorescence intensity as a function of pH, chemical denaturant, or exogenous ligands. The syringes can be operated independently to control the titration volumes and are fitted with a valve for automated refilling and flushing during extended runs. The titration application program in Spectra Manager controls the autotitrator and automatically corrects for concentration during measurement.

Model ATS-826 ATS-827
Compatible Cells Micro cell 5 x 5 mm, rectangular cell 10 x 10 mm
Compatible Accessories STR-811, ETC-814 STR-812, EHC-813, ETC-815
Number of Syringes 2
Syringe Volume Options 1.0 mL (standard), 2.5 mL
Injection Accuracy Greater than 99%
Injection Reproducibility Less than 1%
Injection Pitch 0.1% of syringe volume
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