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LC-4000 Series HPLC Pumps

LC-4000 Series HPLC pumps include expansion bays that allow a simple pump module to be transformed into a powerful solvent delivery system. Accessory modules can be installed in the LC-4000 Series pump for use with virtually any solvent delivery requirement.

HPLC, RHPLC PU-4180
Pumps

Pump Options: Isocratic, Binary and  Quaternary
Flow Rate: Up to 10 mL / min
Operating Pressure: Up to 500 Bar
Degassing: Vacuum (Option)
Solvents: 2, 4, 6 or 10

RHPLC PU-4185
Pumps

Pump Options: Isocratic, Binary and  Quaternary
Flow Rate: Up to 4 mL / min
Operating Pressure: Up to 700 Bar
Degassing: Vacuum (Option)
Solvents: 2, 4, 6 or 10

UHPLC PU-4285
Pumps

Pump Options: Isocratic, Binary and  Quaternary
Flow Rate: Up to 2 mL / min
Operating Pressure: Up to 1,300 Bar
Degassing: Vacuum (Option)
Solvents: 2, 4, 6 or 10

PREP PU-4086/7
Pumps

Pump Options: Isocratic, Binary
Flow Rate: Up to 20 mL / min (PU-4086)
Flow Rate: Up to 50 mL / min (PU-4087)
Operating Pressure: Up to 500 Bar
Degassing: Vacuum (Option)
Solvents: 2, 4, 6 or 10

Upgrade and Expansion

The unique expansion bays in the pump platform can be fitted with plug-in modules to quickly and easily transform a simple isocratic pumping system into a sophisticated multi-solvent delivery system by installing a second pump, degasser, quaternary gradient unit, solvent selection valve or dynamic mixer.
Adding a second HPLC pump module enables an isocratic pump to be upgraded to a dual pump system for either binary gradient or two isocratic flows. The single-unit design of the quaternary gradient solvent delivery system including low internal volume vacuum degasser.

Control

The PU-4000 Series pumps are normally used as part of a complete HPLC system, however for applications that require stand-alone operation, each instrument has its own keypad and can be controlled using a simple RS232 protocol and/or contact closures (TTL) to start and stop the pump.

Eliminating Pulsation

The problems caused by pressure variations in solvent delivery when switching between two pump heads in a double reciprocating pump are virtually eliminated with JASCO’s unique asymmetric design that precisely controls the cross-over points of the plungers. The LC-4000 series analytical pumps offer flow rate precision with RSDs of better than 0.05% (difficult to achieve with commercially available pumps).

Maximum Pressure

The PU-4180 and 4185 pumps operate at pressures up to 70 MPa (700bar), typically used with RHPLC for core-shell columns or similar micro packing materials as well as for use with conventional HPLC. The PU-4280/85 UHPLC pumps operate at pressures up to 130 MPa (1300 bar) for columns with particle sizes of less than 2 μm.

HPLC Pump Configurations

The PU-4180/85 and PU-4280/85 can be configured for single pump gradient and multiple pump gradient operation. The single pump quaternary gradient module offers excellent gradient formation for up to four solvents. A newly designed 4-way valve for switching the solvents is synchronized to the operation of the pump motor control – ‘variable cycle adaption’ optimizes delivery according to gradient resolution and flow rate. This highly efficient quaternary gradient can be applied across a wide flow-rate range. Binary and ternary gradient (using 2 or 3 pumps) with optional built-in degasser can be used for fast cycle high-resolution gradient formation; the gradient response time is superior to the single pump quaternary gradient system.

Solvent Mixing

Multi-pump gradient mixing is done using a variable speed dynamic mixer with a range of a small volume mixing chambers to provide the smallest possible dead volume, offering higher mixing efficiencies for all HPLC solvents. The optional in-line TERA mixer is a turbulent-flow solvent mixer uniquely designed to offer high efficiency mixing with minimal dead volume.

Solvent Degasser

The in-line vacuum solvent degasser uses a Teflon AF membrane with a hold-up volume of only 400μL (per solvent line); for fast solvent priming with minimal waste. Despite its small size, the new degasser offers more effective and stable degassing than larger previous generation models. A degasser for preparative HPLC is also available.

Preparative and Semi-Preparative Pumps

The LC-4000 Preparative-HPLC System includes pumps at three scales. The PU-4086 pump offers flow rates up to 20 mL/min, the PU-4087 pump up to 50 mL/min and the PU-4088 up to 120mL/ min. Both single and multi-pump gradient modes can be used with the PU-4086 pump platform whilst the PU-4087 and PU-4088 are used in multi-pump mode only. The new MK2 technology used in the PU-4086 and PU-4087 motor driver control has been increased so that the maximum pressure can be used at higher flow-rates for all pump models. Solvents can be delivered at higher flow rates and higher pressures for use with longer preparative HPLC columns that generate more back pressure and for faster and more productive preparative separations. Dynamic mixers, solvent selection valves and the prep degasser can be installed in the expansion bays in both the PU-4086 and PU-4087 modules.

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