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SFC Fraction Collection

Jasco HPLC System Building

 

 

 

 

 

MCS-6 ANALYTICAL SFC AND SEMI-PREP SFC – MICROCYCLONE FRACTION COLLECTION


The new MCS-6 MicroCyclone SFC fraction collection system has been designed and patented to suit all fraction collection requirements up to 30mL/min. It matches perfectly to our A-2080 analytical and SP-2086 semi-prep scale systems.

Features
• Patented disposable MicroCyclones –eliminate cross contamination and carry-over
• Recoveries of nearly 100% for non-volatile samples
• Single or 6-way fraction collection, add multiple banks for more samples
• Simple spring loading of sample tubes for easy mounting
• Flow rates up to 30mL/min
• Two fraction vial sizes - 40mL or 100mL
 

MCS-6 ANALYTICAL SFC AND SEMI-PREP SFC – MICROCYCLONE FRACTION COLLECTION

Description
Suitable for semi-preparative supercritical fluid chromatography carried out on columns of 4.6 ~ 10mm bore, for separation and purification of up to mg quantities for high yield purification. For sample collection the FC-2088-31 has been introduced as a flat bed collector suitable for semi-preparative SFC, samples are fractionated using the Micro Cyclone Separator (patent pending), and samples are efficiently recovered to convenient size bottles.

The Micro Cyclone Separator (MCS) is made of PFA (copolymer of tetrafluoroethylene-perfluoroalkyl vinyl ether). The Micro cyclone separator itself, is so inexpensive, single-use operation can be considered.

FC-2088-31 Fraction collector for preparative supercritical fluid chromatography

FC-2088-31 for SFC 5-30mL/min mobile phase flow rate. Samples can be collected on: (40mL standard capacity) bottles collected by the XYZ probe. Installation of gas-liquid separator is as easy as placing it in the mouth of the recovery bottle.

FC-2088-31 is installed in a dedicated Dorafuta. The Dorafuta is equipped with a fan, to force the carbon dioxide to exhaust. The transparent acrylic enclosure, makes it easy to the check the status of the recovery

With other collection devices it can be a problem collecting the sample eluted in a solution or the mobile phase as the rapid volume expansion of the vaporized carbon dioxide (about 500 times the volume ratio) can cause scattering of the collected solution.

The table below shows (chiral flavanone) comparison collected by using a Micro Cyclone Separator and also collection by inserting the pipe in the recovery bottle.

The micro cyclone separator can significantly improve the recovery rate and in addition, you can see from the chromatogram shown the results of the purity of each fraction, sufficient optical purity has been obtained. At the same time, it also helps prevent contamination by preventing sample spitting around the bottle.